It happened in the 4000 block of Oley Turnpike Road in Exeter as a line of severe storms hit the region midday Friday.
Crews arrived to find the tree was uprooted and fell onto the front of the home with a woman and two children inside.
The woman and one of those children were injured. They were taken to the hospital with non-life-threatening injuries.
A dog was also inside the home but was unscathed.
The house is currently uninhabitable, firefighters say.